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Registros recuperados: 11 | |
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Chagas,Edvan Alves; Pasqual,Moacir; Ramos,José Darlan; Cardoso,Pollyana; Cazetta,Jairo Osvaldo; Figueiredo,Milene Alves de. |
This research was undertaken to study the influence of different concentrations of the MT medium, sucrose, vitamins, activated charcoal and gibberellic acid (GA3) on the culture of immature embryos from the crossing between 'Pêra Rio' sweet orange and 'PONCÃ' mandarin. The embryos were excised under aseptic conditions and inoculated in 15 mL of the MT medium according to the following experiments: 1) MT concentrations (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 2) vitamins concentrations of the MT (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 3) activated charcoal concentrations (0, 0.5, 1, 1.5 and 2 g.L-1) supplemented with GA3 (0, 0.01, 0.1; 1 and 10 mg.L-1). After the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo culture; Tissue culture; Biotechnology; Citrus breeding. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452003000300031 |
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López Estrada, Martha Elena. |
La deformación del mango o “escoba de bruja” es una enfermedad de importancia económica que se encuentra presente en México y en otros países productores. Como agentes causales se ha propuesto a hongos del genero Fusarium y se ha sugerido que el ácaro Aceria mangiferae es portador de dicho hongo. Sin embargo, la discusión persiste respecto a la etiología de la enfermedad y no está claramente definido el papel del ácaro; de igual forma, los síntomas en brotes vegetativos e inflorescencias tienen importantes variaciones, por lo que el presente trabajo se realizó con el objetivo de generar información sobre el papel del ácaro en la deformación del mango en su etapa floral y vegetativa. Para ello, plantas de mango sembradas in vitro en condiciones de... |
Tipo: Tesis |
Palavras-chave: Cultivo de embriones; In vitro; Mangifera indica; Fusarium subglutinans; Eriophyidae; Doctorado; Entomología y Acarología; Embryo culture. |
Ano: 2008 |
URL: http://hdl.handle.net/10521/1362 |
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Pasqual,Moacir; Dutra,Leonardo Ferreira; Araujo,Aparecida Gomes de; Figueiredo,Milene Alves de. |
The aim of this work was to evaluate the effect of 'Ponkan' mandarin (C. reticulata) x 'Pêra' sweet orange (C. sinensis) immature fruits storage and sucrose concentrations on embryos in vitro culture. Fruits with 3 to 4 cm in diameter were harvested and placed inside black polyethylene bags with lateral openings and stored at 5±1ºC during 135 days. Every 15 days a sample was removed, its embryos were excised and individually inoculated in test tubes containing 15 mL of MS medium (Murashige & Skoog, 1962) with sucrose (0, 1.5, 3, 6, 12, 18 and 24 g L-1) and 0.3 mg L-1 GA3 and 1 g L-1 activated charcoal. Those treatments rested 48 hours in the dark and later in a growth room at 27 ± 1ºC with a 16-h photoperiod and 32 µmol m-2 s-1 light intensity.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo culture; Nutrient medium; Breeding. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000500029 |
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Figueiredo,Júnia Rafael Mendonça; Paiva,Patrícia Duarte de Oliveira; Silva,Diogo Pedrosa Corrêa da; Paiva,Renato; Souza,Rafaela Ribeiro; Reis,Michele Valquíria dos. |
ABSTRACT Tropical species may require higher temperatures as well as higher growth regulator concentrations for in vitro development. Since these conditions may affect plant metabolism, the objective of this study was to identify how different temperatures and gibberellin concentrations may affect the in vitro development of strelitzia embryos, analyzing the effect on ROS and cytogenetic stability. Zygotic embryos were cultivated on MS medium supplemented with 5, 10 and 20 µM GA3 under temperatures of 25 °C, 30/25 °C and 30 °C. After 60 days, higher embryonic germination rate (72%) and shoot length of plantlets (3.14 cm) were observed on medium containing 20 µM gibberellic acid (GA3). At this concentration, there was an increase in nitrate reductase... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidant; Embryo culture; Gibberellin; Nitrate reductase.. |
Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100204 |
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Registros recuperados: 11 | |
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