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Registros recuperados: 11
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Adaptability and leaf anatomical features in oil palm seedlings produced by embryo rescue and pre-germinated seeds Braz. J. Plant Physiol.
Luis,Zanderluce G.; Bezerra,Kadja Milena G.; Scherwinski-Pereira,Jonny Everson.
Changes in the leaf structure of plants grown in different conditions have been reported, such as increase in size and density of stomata and reduction in stomatal control, amount of epicuticular wax, and mesophyll thickness, with a high diversity of intercellular spaces. However, these changes are highly variable depending on the physiological and morphological characteristics of each species. The objective of this work was to analyze the adaptability and anatomical plasticity of oil palm seedlings produced after embryo rescue and pre-germinated seeds. Expanded leaves were prepared for evaluation of morphometric data and anatomical structures. It was verified that the environmental conditions in vitro negatively influenced the stomata density, epidermal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Elaeis guineensis; Arecaceae; Germination; Embryo culture; Acclimatization; Plantlets metabolism.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000300008
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Development of globular embryos from the hybridization between 'Pêra Rio' sweet orange and 'Poncã' mandarin Rev. Bras. Frutic.
Chagas,Edvan Alves; Pasqual,Moacir; Ramos,José Darlan; Cardoso,Pollyana; Cazetta,Jairo Osvaldo; Figueiredo,Milene Alves de.
This research was undertaken to study the influence of different concentrations of the MT medium, sucrose, vitamins, activated charcoal and gibberellic acid (GA3) on the culture of immature embryos from the crossing between 'Pêra Rio' sweet orange and 'PONCÃ' mandarin. The embryos were excised under aseptic conditions and inoculated in 15 mL of the MT medium according to the following experiments: 1) MT concentrations (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 2) vitamins concentrations of the MT (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 3) activated charcoal concentrations (0, 0.5, 1, 1.5 and 2 g.L-1) supplemented with GA3 (0, 0.01, 0.1; 1 and 10 mg.L-1). After the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo culture; Tissue culture; Biotechnology; Citrus breeding.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452003000300031
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El papel de Ácaro Aceria magniferae (Sayed) en la deformación del Mango o "Escoba de Bruja" Colegio de Postgraduados
López Estrada, Martha Elena.
La deformación del mango o “escoba de bruja” es una enfermedad de importancia económica que se encuentra presente en México y en otros países productores. Como agentes causales se ha propuesto a hongos del genero Fusarium y se ha sugerido que el ácaro Aceria mangiferae es portador de dicho hongo. Sin embargo, la discusión persiste respecto a la etiología de la enfermedad y no está claramente definido el papel del ácaro; de igual forma, los síntomas en brotes vegetativos e inflorescencias tienen importantes variaciones, por lo que el presente trabajo se realizó con el objetivo de generar información sobre el papel del ácaro en la deformación del mango en su etapa floral y vegetativa. Para ello, plantas de mango sembradas in vitro en condiciones de...
Tipo: Tesis Palavras-chave: Cultivo de embriones; In vitro; Mangifera indica; Fusarium subglutinans; Eriophyidae; Doctorado; Entomología y Acarología; Embryo culture.
Ano: 2008 URL: http://hdl.handle.net/10521/1362
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Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars PAB
Ochoa,Elisa del Carmen Martínez; Andrade-Rodríguez,María; Rodríguez,Mario Rocandio; Monter,Angel Villegas.
The objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mangifera indica; Embryo culture; Funiculus; Molecular markers; Polymorphism.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012001100010
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In vitro culture from mature seeds of Passiflora species Scientia Agricola
Guzzo,Flavia; Ceoldo,Stefania; Andreetta,Filippo; Levi,Marisa.
The genus Passiflora comprises hundred species, mainly native of the South American tropics and rainforests, which are grouped into 21 subgenera. Some species are widely studied for their economic importance and are chiefly cultivated for production of fruit juice. To obtain a continuous source of material for a screening of secondary metabolites, zygotic embryo culture was attempted for 62 Passiflora species, starting from seeds mainly collected in the wild. Twenty nine of these species produced calli, which had very different growth rates. Plants were successfully regenerated from calli of 13 different species. For 25 of the responsive species this is the first report of in vitro culture.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passiflora regeneration; Embryo culture; Endosperm culture; Ethnobotanical species; Plant acclimatization.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000100018
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In Vitro Propagation of Heliconia bihai (L.) L. from Zygotic Embryos Acta Botanica
Ulisses,Cláudia; Melo-de-Pinna,Gladys Flávia; Willadino,Lilia; Albuquerque,Cynthia Cavalcanti de; Camara,Terezinha Rangel.
The internal morphology of embryos from immature and mature fruits of Heliconia bihai (L.) L. cv. Lobster Claw Two was examined. Embryos were inoculated into MS media (full MS and ½ MS) and GA3 (0, 2.5 and 5 mg L-1) with either sucrose or glucose. These plantlets were then replicated and transferred to MS medium (full MS or ½ MS) with 0 or 2.5 mg L-1 BAP and their multiplication was evaluated 30 and 45 days after inoculation. The genetic variability of the multiplied plants was estimated using isoenzyme analyses. The internal morphology of the mature embryos revealed their tissues to be in more advanced stages of differentiation than immature embryos. In the conversion phase, 85% of the inoculated embryos developed into plants in the ½ MS medium with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrates; Embryo culture; Histology; Isoenzymes; Micropropagation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062010000100018
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Influences of medium and seed age on callus formation from mature embryos of wild rice (Zizania palustris L.) OAK
村山, 廉生; 三浦, 秀穂; MURAYAMA, Yasuo; MIURA, Hideho.
Palavras-chave: 遺伝子源; カルス誘導; 胚培養; ワイルドライス; Gene resource; Callus formation; Embryo culture; Wild rice.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1338
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Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD PAB
Andrade-Rodríguez,María; Villegas-Monter,Angel; Carrillo-Castañeda,Guillermo; García-Velázquez,Armando.
The objectives of this work were to evaluate the frequency of polyembryony, and to identify zygotic and nucellar seedlings of Citrus volkameriana using RAPD. Twenty-five polyembryonic and eight monoembryonic seeds were cultivated in vitrofor six months. DNA from seedlings was extracted and used in combination with five RAPD primers to identify zygotic or nucellar origin of the seedlings. Environmental conditions of the year affected significantly (P<0.05) the morphological characteristics of fruitsand the number ofembryos per seed. Polyembryonic seeds ranged from 30.9%, 44.8% to 54.4% over three years. Morphological characteristic was not correlated with polyembryony. In vitro culture enable all embryos of each seed to grow, favoring the percentage of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus volkameriana; Rootstock; Embryo culture; Molecular markers; Polymorphism.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000600006
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'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage Ciência e Agrotecnologia
Pasqual,Moacir; Dutra,Leonardo Ferreira; Araujo,Aparecida Gomes de; Figueiredo,Milene Alves de.
The aim of this work was to evaluate the effect of 'Ponkan' mandarin (C. reticulata) x 'Pêra' sweet orange (C. sinensis) immature fruits storage and sucrose concentrations on embryos in vitro culture. Fruits with 3 to 4 cm in diameter were harvested and placed inside black polyethylene bags with lateral openings and stored at 5±1ºC during 135 days. Every 15 days a sample was removed, its embryos were excised and individually inoculated in test tubes containing 15 mL of MS medium (Murashige &amp; Skoog, 1962) with sucrose (0, 1.5, 3, 6, 12, 18 and 24 g L-1) and 0.3 mg L-1 GA3 and 1 g L-1 activated charcoal. Those treatments rested 48 hours in the dark and later in a growth room at 27 ± 1ºC with a 16-h photoperiod and 32 µmol m-2 s-1 light intensity....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo culture; Nutrient medium; Breeding.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000500029
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Produção in vivo e recuperação não cirúrgica de embriões ovinos no Brasil. Infoteca-e
FONSECA, J. F. da.
Esta circular técnica sumariza resultados de produção in vivo de embriões ovinos coletados especificamente pela via transcervical (NSER) no Brasil, que dão suporte à recomendação do Protocolo Embrapa de Superovulação em várias raças ovinas.
Tipo: Folhetos Palavras-chave: Ovino; Reprodução Animal; Cultura de Embrião; Superovulação; Sheep; Animal reproduction; In vitro culture; Embryo culture; Brazil.
Ano: 2021 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1137612
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Temperature and GA3 on ROS and cytogenetic stability during in vitro cultivation of strelitzia zygotic embryos Ciência e Agrotecnologia
Figueiredo,Júnia Rafael Mendonça; Paiva,Patrícia Duarte de Oliveira; Silva,Diogo Pedrosa Corrêa da; Paiva,Renato; Souza,Rafaela Ribeiro; Reis,Michele Valquíria dos.
ABSTRACT Tropical species may require higher temperatures as well as higher growth regulator concentrations for in vitro development. Since these conditions may affect plant metabolism, the objective of this study was to identify how different temperatures and gibberellin concentrations may affect the in vitro development of strelitzia embryos, analyzing the effect on ROS and cytogenetic stability. Zygotic embryos were cultivated on MS medium supplemented with 5, 10 and 20 µM GA3 under temperatures of 25 °C, 30/25 °C and 30 °C. After 60 days, higher embryonic germination rate (72%) and shoot length of plantlets (3.14 cm) were observed on medium containing 20 µM gibberellic acid (GA3). At this concentration, there was an increase in nitrate reductase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; Embryo culture; Gibberellin; Nitrate reductase..
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100204
Registros recuperados: 11
Primeira ... 1 ... Última
 

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